Just Passing Through

Transmitted light microscopy is a type of light microscopy where light is transmitted, or passed through, a sample. For this type of light microscopy samples are required to allow light through them. A Transmitted light microscope uses light that passes through a condenser into an adjustable aperture then through the sample into a series of lenses to the eyepiece. The condenser and condenser aperture combination controls the light in a way that gives illumination that allows for the right balance of resolution and contrast. This type of microscopy is used to distinguish morphological characteristics and optical properties of samples. There are several different techniques for transmitted light that are used for sample analysis:

  • Bright-field microscopy
  • Dark-field microscopy
  • Phase contrast microscopy
  • Polarization microscopy
  • Differential interference contrast optics microscopy

Transmission light microscopes are used to look at thin sections – the specimen must transmit the light which includes tissues, cell walls, crystalline components, and thin films, etc.


Related standards

  • ASTM
  • CE
  • Environmental Protection Agency
  • European Union
  • Food & Drug Administration
  • ICH Q3D
  • International Council for Harmonisation
  • International Organization for Standardization

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