Is your compound extremely polar? Use HILIC!

HILIC is one of the most successful techniques for the separation and quantitation of polar organic compounds. It is most commonly applied for molecules which are too polar to be retained by reverse phase chromatography.  In HILIC, the stationary phase is strongly polar (typically ionic, polyamide or silica) and uses a high percentage of organic solvent (acetonitrile). This results in formation of an enriched water layer around the stationary phase. Partitioning between the water layer and the bulk mobile phase leads to retention and causes separation of sample components.

  • Retention of hydrophilic compounds that are difficult to retain by Reversed Phase Liquid Chromatography
  • Efficient separation of very polar analytes

We are passionate about your success. Tell us more about your regulatory and quality needs to learn about how we can help.

Book a Consultation


To display custom copy instead of global copy in this section, please go to Show Global Content for Bottom CTA? toggle in the "Contents" tab to the left, toggle it off, save, and then REFRESH the page editor, the custom text will then show up and ready to be edited.

Turning the global content back on will be the same process, go to the toggle and toggle it back on, save and refresh!