Dynamic light scattering (DLS) is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles in the sub-micron range. Typical applications of dynamic light scattering are the characterization of particles, emulsions or droplets which have been dispersed or dissolved in a liquid. The Brownian motion of particles or molecules in suspension causes laser light to be scattered at different intensities. The larger the particle, the slower the Brownian motion will be. Analysis of these intensity fluctuations yields the velocity of the Brownian motion and hence the particle size using the Stokes-Einstein relationship. DLS provides a value that refers to how a particle diffuses within a fluid so it is referred to as a hydrodynamic diameter.
Variables that affect the DLS measurements are as follows:
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