Bis(2,4-di-tert-butylphenyl)-phosphate (bDtBPP) has been the subject of much discussion in the area of Extractables and Leachables testing for single use bioprocess systems.

It has been shown to inhibit cell growth at low levels (ppm range). It was found that when oxidized Irgafos 168 is subjected to gamma irradiation, this compound is formed, which can leach and kills cells in a bioprocess system. Irgafos 168 is very common in polyolefins.  For more information, please see

RQM+ Lab Services has helped companies identify and quantitate bis(2,4-di-tert-butylphenyl)-phosphate and many other compounds because we have an extensive proprietary databases of extractable compounds.

1. Identification of a leachable compound detrimental to cell growth in single-use bioprocess containers. (Hammond M, Nunn H, Rogers G, Lee H, Marghitoiu AL, Perez L, Nashed-Samuel Y, Anderson C, Vandiver M, Kline S.)


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